RESUMEN
PURPOSE: To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod imunoexpression in a model of dorsal burn in rats. METHODS: Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry. RESULTS: The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher. CONCLUSION: Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression. .
Asunto(s)
Animales , Masculino , Quemaduras/radioterapia , Terapia por Luz de Baja Intensidad/métodos , Músculo Esquelético/efectos de la radiación , Proteína MioD/análisis , Quemaduras/metabolismo , Quemaduras/patología , Recuento de Células , Modelos Animales de Enfermedad , Inmunohistoquímica , Fibras Musculares Esqueléticas/efectos de la radiación , Músculo Esquelético/patología , Proteína MioD/efectos de la radiación , Ratas Wistar , Reproducibilidad de los Resultados , Piel/lesiones , Piel/efectos de la radiación , Factores de Tiempo , Resultado del TratamientoRESUMEN
Embryonal rhabdomyosarcoma is the most common soft tissue sarcoma in children. We report a rare case of embryonal rhabdomyosarcoma of the soft palate in a 32-year-old Caucasian female. Detailed histology of the tumor is described. Positive staining with desmin, myogenin and myoD1 confirmed the tumor to be embryonal rhabdomyosarcoma. A genetic association between rhabdomyosarcoma, polycystic ovary syndrome and the FEM1A gene on the human chromosome is speculated upon.
Asunto(s)
Adulto , Desmina/análisis , Población Blanca , Femenino , Cabeza/diagnóstico por imagen , Histocitoquímica , Humanos , Inmunohistoquímica , Imagen por Resonancia Magnética , Microscopía , Neoplasias de la Boca/diagnóstico , Neoplasias de la Boca/patología , Proteína MioD/análisis , Miogenina/análisis , Paladar Blando/patología , Rabdomiosarcoma Embrionario/diagnóstico , Rabdomiosarcoma Embrionario/patologíaRESUMEN
Non-hematopoietic malignancies infiltrating bone marrow have always been a source of erroneous diagnosis. Among these, the small round cell tumors like neuroblastomas and rhabdomyosarcomas mimick the hematopoietic blasts. Several case reports of rhabdomyosarcoma mimicking acute leukemia, clinically and morphologically at presentation have been reported in the literature. To the best of our knowledge such an entity has not been reported in Indian literature. We report here one such case of alveolar rhabdomyosarcoma masquerading as acute leukemia. A thorough clinical examination with high degree of suspicion on bone marrow morphology and judicious use of appropriate immunohistochemistry markers will solve many of these cases.